African green monkey kidney cell lines (Vero) were cultured in Minimum Essential Media (MEM; biowest, Nuaillé, France) supplemented with 10 mM HEPES (Gibco, Carlsbad, CA, USA) and 10% antibiotic-antimycotic (Gibco, Carlsbad, CA, USA) fetal bovine serum (Gibco, Carlsbad, CA, USA) at 37 °C with 5% CO2. In this study, we used the PEDV strain PED-CUP-B2014 [30 (link)]. Virus titer was determined by TCID50 assay [31 (link)].
Free full text: Click here