Characterization of MTX-Loaded ZnO Nanoparticles

MTX-ZnONPs were characterized by UV-vis spectrophotometry (Shimadzu dual-beam spectrophotometer, model UV-1601 PC, 1 nm resolution. Kyoto, Japan). The mean particle size and zeta potential of MTX-ZnONPs were determined using a particle size analyzer (Zetasizer Nano-ZS, Model ZEN3600, Malvern Instruments Ltd., Malvern, United Kingdom) [(Gomathy and Sabarinathan, 2010 ), 15]. Transmission electron microscope (Tecnai™ G2 Spirit Bio-TWIN, FEI, Hillsboro, United States) was used to investigate the size of the inorganic core at a accelerating voltage of 80 kV (Khan S. et al., 2018 (link)). The binding confirmation of MTX on the surface of ZnONPs was analyzed by Fourier-transform infrared spectroscopy (FTIR) (Shimadzu FTIR-8201 PC infrared spectrophotometer) operating in the diffuse reflectance mode at a resolution of 4 cm⁻¹. A total of 256 scans of the ZnONPs film (400–4,000 cm⁻¹ range) were acquired to obtain good signal-to-noise ratios.

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Mishra P., Ali Ahmad M.F., Al-Keridis L.A., Saeed M., Alshammari N., Alabdallah N.M., Tiwari R.K., Ahmad A., Verma M., Fatima S, & Ansari I.A. (2023). Methotrexate-conjugated zinc oxide nanoparticles exert a substantially improved cytotoxic effect on lung cancer cells by inducing apoptosis. Frontiers in Pharmacology, 14, 1194578.

Publication 2023

UV-1601 PC Tecnai™ G2 Spirit Bio-TWIN FTIR-8201 PC infrared spectrophotometer

Ftir Scans Spectrophotometry Transmission electron microscope Twin

Corresponding Organization :

Other organizations : Integral University, Saudi Arabia Basic Industries (Saudi Arabia)

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Variable analysis

independent variables

None explicitly mentioned

dependent variables

UV-vis spectrophotometry to characterize MTX-ZnONPs
Particle size and zeta potential of MTX-ZnONPs determined using a particle size analyzer
Size of the inorganic core investigated using Transmission Electron Microscope (TEM)
Binding confirmation of MTX on the surface of ZnONPs analyzed by Fourier-transform infrared spectroscopy (FTIR)

control variables

None explicitly mentioned

positive controls

None mentioned

negative controls

None mentioned

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