Brain Tumor Immunofluorescence Analysis in Boxer Dog

The MRI of a boxer dog having spontaneously developed a brain tumor was performed at the Veterinary School of Maisont-Alfort (94-France) by Dr. P. Devauchelle and tumor samples were obtained with the consent of the dog owner. For immunofluorescence staining, cells were processed as previously described (20 (link), 30 (link)). Microscopy was performed at room temperature with the Leica SP5 confocal system, attached to a DMI6000 stand, with a 636/1.4 oil immersion objective. Images were processed with the freely available ImageJ software (http://rsb.info.nih.gov.gate1.inist.fr/ij/) and the Leica SP5 confocal system.

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Biau J., Chautard E., Berthault N., de Koning L., Court F., Pereira B., Verrelle P, & Dutreix M. (2019). Combining the DNA Repair Inhibitor Dbait With Radiotherapy for the Treatment of High Grade Glioma: Efficacy and Protein Biomarkers of Resistance in Preclinical Models. Frontiers in Oncology, 9, 549.

Publication 2019

Leica SP5 confocal system

Brain tumor Cells Immersion Immunofluorescence Microscopy Tumor

Corresponding Organization : Université Paris-Sud

Other organizations : Centre Hospitalier Universitaire de Clermont-Ferrand

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Variable analysis

independent variables

None explicitly mentioned

dependent variables

None explicitly mentioned

control variables

Temperature (room temperature)
Microscope (Leica SP5 confocal system, DMI6000 stand)
Objective (63x/1.4 oil immersion)

controls

Positive control: Not specified
Negative control: Not specified

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