B-HA and S-HA scaffolds were analyzed using scanning electron microscopy (SEM). Samples were washed with saline, fixed by 2.5% glutaraldehyde and with a 1% osmium solution in phosphate buffer. The specimens were coated with colloidal gold and analyzed using scanning electron microscopy (SEM, model Stereoscan S-360, Cambridge UK) [41 (link)].
The open and total porosity of the studied ceramics was measured using Archimedes’ method and geometrical weight-volume evaluation, respectively. The specific surface area (SSA) of the scaffold was measured using the nitrogen adsorption method, following the Brunauer–Emmett–Teller (BET) model (Sorpty 1750, Carlo Erba, Milan, Italy).
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