B-HA and S-HA scaffolds were analyzed using scanning electron microscopy (SEM). Samples were washed with saline, fixed by 2.5% glutaraldehyde and with a 1% osmium solution in phosphate buffer. The specimens were coated with colloidal gold and analyzed using scanning electron microscopy (SEM, model Stereoscan S-360, Cambridge UK) [41 (link)].
The open and total porosity of the studied ceramics was measured using Archimedes’ method and geometrical weight-volume evaluation, respectively. The specific surface area (SSA) of the scaffold was measured using the nitrogen adsorption method, following the Brunauer–Emmett–Teller (BET) model (Sorpty 1750, Carlo Erba, Milan, Italy).
The open and total porosity of the studied ceramics was measured using Archimedes’ method and geometrical weight-volume evaluation, respectively. The specific surface area (SSA) of the scaffold was measured using the nitrogen adsorption method, following the Brunauer–Emmett–Teller (BET) model (Sorpty 1750, Carlo Erba, Milan, Italy).
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