Hemolymphs of 50 infected D. citri insects were collected and then were placed on Polysine adhesion slides (Thermo Fisher Scientific) and dried at room temperature. The samples were fixed in 4% paraformaldehyde for 2 h and treated with 0.2% Triton X-100 for 1 h as previously described (12 (link)) and then were immunolabeled with P8-FITC or actin dye phalloidin-Alexa Fluor 647 carboxylic acid (Thermo Fisher Scientific). The treated samples were examined with a Leica TCS SPE inverted confocal microscope.
Organs of D. citri insects were successively dissected, fixed, and treated with Triton X-100, as mentioned above. The samples then were immunolabeled with P10-FITC and finally detected using immunofluorescence microscopy.
Organs of D. citri insects were successively dissected, fixed, and treated with Triton X-100, as mentioned above. The samples then were immunolabeled with P10-FITC and finally detected using immunofluorescence microscopy.
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