Carbon Source Degradation Analysis

The carbon source degradation products were detected by thin-layer chromatography (TLC) analysis. Briefly, samples (0.2 mL) were loaded onto a pre-coated silica gel-60 TLC aluminum plate (Merck, Darmstadt, Germany). After being developed with a solvent system consisting of formic acid/n-butanol/water (6:4:1, v:v:v), the plate was soaked in orcinol reagent (Sigma-Aldrich Co., LLC., St. Louis, MO, USA) and visualized at 120 °C for 1 min [8 (link)]. The scanned TLC profiles were analyzed by Quantity One (BioRad, Hercules, CA, USA) and the amounts of carbon source in each sample were quantified. Degradation rates were calculated as the percentage of total amounts at 0 h subtracted from those at 24 h after fermentation.

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Li Y., Jiang J., Zhu L., Wang X., Wan W., Wang D, & Li Z. (2022). Evaluation of Intestinal Microbial Metabolites in Preterm Infants with Different Initial Feeding Methods by In Vitro Fermentation Modeling System. Microorganisms, 10(7), 1453.

Publication 2022

gel-60 TLC aluminum plate Quantity One

Aluminum Butanol n Carbon Fermentation Formic acid Orcinol Silica gel Solvent Thin layer chromatography

Corresponding Organization : Chinese Academy of Medical Sciences & Peking Union Medical College

Other organizations : ZheJiang Academy of Agricultural Sciences

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Variable analysis

independent variables

Solvent system consisting of formic acid/n-butanol/water (6:4:1, v:v:v)

dependent variables

Carbon source degradation products detected by thin-layer chromatography (TLC) analysis
Amounts of carbon source in each sample quantified
Degradation rates calculated as the percentage of total amounts at 0 h subtracted from those at 24 h after fermentation

control variables

Pre-coated silica gel-60 TLC aluminum plate (Merck, Darmstadt, Germany)
Orcinol reagent (Sigma-Aldrich Co., LLC., St. Louis, MO, USA) used for visualization
Visualization at 120 °C for 1 min

controls

No positive or negative controls were explicitly mentioned in the protocol.

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