Co-Immunoprecipitation and Western Blotting Protocol

Co-immunoprecipitation and Western blotting were performed as described previously [16 (link)]. Primary antibodies used included: anti-FOXM1 (1:500, Santa Cruz (SC), #271746, 1:500, Abcam, #207298), anti-pALK Y1604 (1:500, Cell Signaling technologies (CST), #3341S), anti-ALK (1:1000, CST, #3633), anti-pSTAT3 (Y705) (1:2000, CST, #9145), anti-STAT3 (1:1000, CST, #124H6), anti-Cyclin B1 (1:1000, SC, #752), anti-β-Actin (1:8000, SC, #47778), anti-PARP (1:1000, CST, #9542), anti-Caspase 3(1:1000, CST, #9662), anti-Survivin (1:1000, CST, #2808), anti-NPM1 (1:2000, Milipore Sigma, clone 3C9), anti-Vinculin (1:500, SC, #25336), anti HDAC-1 (1:500, SC, #81598), and anti-α-Actinin (1:500, SC, 17829). Secondary antibodies used were HRP-conjugated anti-mouse (1:2000, CST, #7076) and anti-rabbit (1:2000, CST, #7074). Detailed information of western blot can be found at Figure S5.