Isolation and Characterization of ECFCs

Endothelial Colony Forming Cells (ECFCs) were isolated from > 50 ml human umbilical cord blood (UCB) of healthy newborns, as described previously [14 (link), 15 (link)], after maternal informed consent and in compliance with Italian legislation, and analyzed for the expression of surface antigens (CD45, CD34, CD31, CD105, ULEX, vWF, KDR, uPAR) by flow-cytometry [14 (link)]. ECFCs were grown in EGM-2 culture medium (Lonza), supplemented with 10% FBS (Euroclone) onto gelatin coated dishes. Human microvascular endothelial cells (HMVEC) were purchased from Lonza and were grown in the same conditions of ECFCs.

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Chillà A., Margheri F., Biagioni A., Del Rosso M., Fibbi G, & Laurenzana A. (2018). Mature and progenitor endothelial cells perform angiogenesis also under protease inhibition: the amoeboid angiogenesis. Journal of Experimental & Clinical Cancer Research : CR, 37, 74.

Publication 2018

EGM-2 culture medium FBS

Culture medium Dishes Endothelial cells Flow cytometry Gelatin Human Maternal Surface antigens Ulex Umbilical cord blood Upar

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Variable analysis

independent variables

None explicitly mentioned

dependent variables

Expression of surface antigens (CD45, CD34, CD31, CD105, ULEX, vWF, KDR, uPAR) on Endothelial Colony Forming Cells (ECFCs)

control variables

Isolation of ECFCs from > 50 ml human umbilical cord blood (UCB) of healthy newborns
Growth of ECFCs in EGM-2 culture medium (Lonza), supplemented with 10% FBS (Euroclone) onto gelatin coated dishes
Growth of Human microvascular endothelial cells (HMVEC) in the same conditions as ECFCs

controls

Positive control: Not explicitly mentioned
Negative control: Not explicitly mentioned

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