EV pellets were fixed with 4% paraformaldehyde (PFA). Following a total of 8 washes using PBS, grids were contrasted with a uranyl-oxalate solution for 5 minutes and transferred to methyl-cellulose-uranyl acetate for 10 minutes on ice as previously described [16 (link)]. Samples were examined on a JEOL JEM-1220 transmission electron microscope (TEM) (JEOL USA, Inc.).
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