Compound 4 was prepared from 3 using LgdB1 [13 (link)]. A 125-mL reaction mixture containing 2.0 g of 3 (not completely dissolved at the start of the reaction due to its low solubility) and 0.36 mg LgdB1 in 10 mM Tris-HCl buffer (pH 7.5) was rotary-shaken (150 rpm) at 30 °C for 18 h. After the reaction, His6-tagged LgdB1 was removed by adding 1 mL of Nuvia IMAC Ni-charged Resin (Bio-Rad Laboratories, Inc. Hercules, CA, USA) followed by filtration. The mixture was deionized by electrodialysis using a Microacylyzer S1 with an AC220-10 membrane cassette (Astom Corporation, Tokyo, Japan), concentrated to approximately 20 mL using a rotary evaporator, and lyophilized to obtain 1.89 g of 4 (yield 94 %).
Protocol detail
Enzymatic Synthesis of Compound 4
Partial Protocol Preview
This section provides a glimpse into the protocol.
The remaining content is hidden due to licensing restrictions, but the full text is available at the following link:
Access Free Full Text.
Corresponding Organization :
Other organizations : Niigata University, The University of Tokyo, Shizuoka University
Top 5 similar protocols
Variable analysis
independent variables
- Amount of compound 3 (2.0 g)
- Concentration of LgdB1 enzyme (0.36 mg)
- Yield of compound 4 (1.89 g, 94%)
- Reaction temperature (30 °C)
- Reaction time (18 h)
- Reaction buffer (10 mM Tris-HCl, pH 7.5)
- Agitation speed (150 rpm)
- Positive control: Not explicitly mentioned
- Negative control: Not explicitly mentioned
Annotations
Based on most similar protocols
Etiam vel ipsum. Morbi facilisis vestibulum nisl. Praesent cursus laoreet felis. Integer adipiscing pretium orci. Nulla facilisi. Quisque posuere bibendum purus. Nulla quam mauris, cursus eget, convallis ac, molestie non, enim. Aliquam congue. Quisque sagittis nonummy sapien. Proin molestie sem vitae urna. Maecenas lorem.
Sign up for free or login to display all annotations
As authors may omit details in methods from publication, our AI will look for missing critical information across the 5 most similar protocols.
About PubCompare
Our mission is to provide scientists with the largest repository of trustworthy protocols and intelligent analytical tools, thereby offering them extensive information to design robust protocols aimed at minimizing the risk of failures.
We believe that the most crucial aspect is to grant scientists access to a wide range of reliable sources and new useful tools that surpass human capabilities.
However, we trust in allowing scientists to determine how to construct their own protocols based on this information, as they are the experts in their field.
Ready to get started?
Sign up for free.
Registration takes 20 seconds.
Available from any computer
No download required
Revolutionizing how scientists
search and build protocols!