All the primers used in this study were purchased from Thermo Fisher, gene blocks were purchased from IDT DNA, and restriction enzymes were purchased from New England Biolabs. The primary antibodies used in this study are the following: mouse α-V5 (clone SV5-Pk2, BioRad); rabbit α-V5 (ICL, RV5-45A-Z), rat α-hemagglutinin (HA, clone 3F10, Sigma); mouse α-tubulin (clone B-5-1-2, Sigma); rabbit α-Histone H3 (ab1971, Abcam); mouse α-Centrin (CrCen clone 20H5, EMD Millipore); and rabbit α-PfGAP45 (gift from Julian Rayner at the University of Cambridge [53 (link)]). Secondary antibodies and other reagents (Alexa Fluor 405 NHS-Ester, SYTOX Deep Red Nucleic acid stain, and Hoechst 33342 solution) used for microscopy were purchased from Thermo Fisher.
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Antibody Reagents for Microscopy Experiments
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Corresponding Organization : Boston Children's Hospital
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- Primers purchased from Thermo Fisher
- Gene blocks purchased from IDT DNA
- Restriction enzymes purchased from New England Biolabs
- Unclear based on the information provided
- Mouse α-V5 (clone SV5-Pk2, BioRad)
- Rabbit α-V5 (ICL, RV5-45A-Z)
- Rat α-hemagglutinin (HA, clone 3F10, Sigma)
- Mouse α-tubulin (clone B-5-1-2, Sigma)
- Rabbit α-Histone H3 (ab1971, Abcam)
- Mouse α-Centrin (CrCen clone 20H5, EMD Millipore)
- Rabbit α-PfGAP45 (gift from Julian Rayner at the University of Cambridge [53 (link)])
- Alexa Fluor 405 NHS-Ester, SYTOX Deep Red Nucleic acid stain, and Hoechst 33342 solution purchased from Thermo Fisher
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