Histologic Tissue Analysis with IHC

Tissues were fixed in zinc-formalin, paraffin-embedded, sectioned at 5 µm and stained with hematoxylin and eosin (H&E) or prepared for immunostaining as described (Hao et al. 2018 (link)). Images were captured with 10×, 20×, or 40× objectives using a Nikon Eclipse NI-U with a DS-QI1 or DS-Ri1 camera and NIS Elements software and adjusted in Adobe Photoshop. For IF, antibodies were as follows: rabbit anti-Acss2 (Abcam, 66038), mouse anti-TGIF1 (Santa Cruz Biotechnology, SC-17000), rabbit anti-Acat1 (Proteintech, 16215-1-AP), rabbit anti-Slc2a1 (Millipore 07-1401), and mouse anti-β-catenin (BD Transduction Labs, 610153).

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Shah A., Melhuish T.A., Fox T.E., Frierson HF J.r, & Wotton D. (2019). TGIF transcription factors repress acetyl CoA metabolic gene expression and promote intestinal tumor growth. Genes & Development, 33(7-8), 388-402.

Publication 2019

Eclipse NI-U DS-QI1 DS-Ri1 mouse anti-β-catenin

Acss2 Antibodies Eosin Formalin Mouse Paraffin Rabbit Tissues Zinc β catenin

Corresponding Organization :

Other organizations : University of Virginia

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Variable analysis

independent variables

Tissue fixation in zinc-formalin
Paraffin-embedding
Sectioning at 5 µm
Staining with hematoxylin and eosin (H&E) or immunostaining

dependent variables

Microscopic images captured with 10×, 20×, or 40× objectives
Image adjustments in Adobe Photoshop

control variables

Nikon Eclipse NI-U microscope with DS-QI1 or DS-Ri1 camera
NIS Elements software

controls

No positive or negative controls were explicitly mentioned.

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