The body mass index (BMI) was determined by measuring the square height (m2) and body weight (kg) followed hemodialysis. [15 (link)]. Before initiating hemodialysis, each patient had a blood sample of about 5 milliliters obtained. An autoanalyzer (Siemens Advia 1800; Siemens Healthcare, Henkestr, Erlangen, Germany) was used to measure the hemoglobin level (Sysmex SP-1000i, Sysmex American, Mundelein, IL, USA) and centrifuge the remaining blood sample for biochemical analyses. The dialysis clearance was measured using the urea kinetic model, which includes the urea reduction ratio and the fractional clearance index for urea (Kt/V). The serum levels of intact parathyroid hormone (iPTH) (Diagnostic Systems Laboratories, Webster, TX, USA) and ANGPTL3 (R&D Systems, Inc., Minneapolis, MN, USA) were measured using commercially available assay kits. [16 (link),17 (link)].
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