The composition of CDM2 has been described previously103 (link),104 (link),123 (link): 50% IMDM + GlutaMAX (Thermo Fisher, 31980-097) + 50% F12 + GlutaMAX (Thermo Fisher, 31765-092) + 1 mg/mL polyvinyl alcohol (Sigma, P8136-250G) + 1% v/v chemically defined lipid concentrate (Thermo Fisher, 11905-031) + 450 μM 1-thioglycerol (Sigma, M6145-100ML) + 0.7 μg/mL recombinant human insulin (Sigma, 11376497001) + 15 μg/mL human transferrin (Sigma, 10652202001) + 1% v/v penicillin/streptomycin (Thermo Fisher, 15070-063).
The composition of CDM3 has been described previously202 (link): 45% IMDM + GlutaMAX (Thermo Fisher, 31980-097) + 45% F12 + GlutaMAX (Thermo Fisher, 31765-092) + 10% KnockOut Serum Replacement (Thermo Fisher, 10828028) + 1 mg/mL polyvinyl alcohol (Sigma, P8136-250G) + 1% v/v chemically defined lipid concentrate (Thermo Fisher, 11905-031) + 1% v/v penicillin/streptomycin (Thermo Fisher, 15070-063).
To prepare CDM2 and CDM3, first polyvinyl alcohol was suspended in either IMDM or F12 by gentle warming and magnetic stirring. After polyvinyl alcohol was dissolved, the remaining media components were mixed together, and the complete media was sterilely filtered through a 0.22 μm filter prior to use.
The composition of CDM3 has been described previously202 (link): 45% IMDM + GlutaMAX (Thermo Fisher, 31980-097) + 45% F12 + GlutaMAX (Thermo Fisher, 31765-092) + 10% KnockOut Serum Replacement (Thermo Fisher, 10828028) + 1 mg/mL polyvinyl alcohol (Sigma, P8136-250G) + 1% v/v chemically defined lipid concentrate (Thermo Fisher, 11905-031) + 1% v/v penicillin/streptomycin (Thermo Fisher, 15070-063).
To prepare CDM2 and CDM3, first polyvinyl alcohol was suspended in either IMDM or F12 by gentle warming and magnetic stirring. After polyvinyl alcohol was dissolved, the remaining media components were mixed together, and the complete media was sterilely filtered through a 0.22 μm filter prior to use.
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