Assessing Bacteremia and Inflammation in Sepsis

Bacteremia was regularly assessed by culture of small blood samples taken from the tail vein as previously described (Steele et al., 2017 (link)). In another experiment, systemic inflammation was assessed by measuring IL-6 in plasma samples obtained from animals sacrificed on day 4 and 2 weeks alongside non-sepsis controls using a high sensitivity ELISA kit (eBIOSCIENCE, Vienna, Austria). TNFα and IL-10 were assessed in non-sepsis controls and animals with sepsis on day 4 and 2 weeks (in the same mice) using Meso Scale Discovery (Rockville, Maryland) customized V-PLEX multiplex assay. These assays were conducted in singlet due to limited sample volumes.

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Owen A.M., Patel S.P., Smith J.D., Balasuriya B.K., Mori S.F., Hawk G.S., Stromberg A.J., Kuriyama N., Kaneki M., Rabchevsky A.G., Butterfield T.A., Esser K.A., Peterson C.A., Starr M.E, & Saito H. (2019). Chronic muscle weakness and mitochondrial dysfunction in the absence of sustained atrophy in a preclinical sepsis model. eLife, 8, e49920.

Publication 2019

high sensitivity ELISA kit

Animals Assays Bacteremia Culture of blood Elisa Il 10 Inflammation Mice Plasma Sensitivity Sepsis Tail Tnfα Vein

Corresponding Organization :

Other organizations : University of Kentucky, Shriners Hospitals for Children - Erie, Harvard University, Massachusetts General Hospital, University of Florida, Markey Cancer Center

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Variable analysis

independent variables

Sepsis condition (sepsis vs. non-sepsis controls)

dependent variables

Bacteremia (assessed by culture of small blood samples)
Systemic inflammation (assessed by measuring IL-6, TNFα, and IL-10 in plasma samples)

control variables

Time points (day 4 and 2 weeks)

controls

Positive control: Non-sepsis controls
Negative control: Not explicitly mentioned

Annotations

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