Histological Ankle Joint Analysis

Ankles were formalin-fixed, decalcified with a decalcifying solution (Sigma-Aldrich), and embedded in paraffin. Ankle joint sections (5 μm) were subjected to hematoxylin and eosin (H&E) and safranin O staining. Slides were imaged using a Nikon microscope imaging system and NIS-Elements software (Nikon Instruments, Tokyo, Japan). The assessment of synovial inflammation, bone erosion, and cartilage damage was conducted as previously described (36 (link)).

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Kim S., Chun S.H., Cheon Y.H., Kim M., Kim H.O., Lee H., Hong S.T., Park S.J., Park M.S., Suh Y.S, & Lee S.I. (2024). Peptoniphilus gorbachii alleviates collagen-induced arthritis in mice by improving intestinal homeostasis and immune regulation. Frontiers in Immunology, 14, 1286387.

Publication 2023

decalcifying solution Nikon microscope imaging system NIS-Elements software

Corresponding Organization : Gyeongsang National University

Other organizations : Jeonbuk National University

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Variable analysis

independent variables

Formalin-fixing of ankles
Decalcification of ankles with decalcifying solution (Sigma-Aldrich)
Embedding of ankles in paraffin

dependent variables

Synovial inflammation
Bone erosion
Cartilage damage

control variables

Ankle joint sections (5 μm)
Hematoxylin and eosin (H&E) and safranin O staining
Imaging using Nikon microscope imaging system and NIS-Elements software

controls

The assessment of synovial inflammation, bone erosion, and cartilage damage was conducted as previously described (36 (link))

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