To quantify Gaussia luciferase (GLuc) activity in infected cell cultures, we used a microplate luminometer (Orion II; Berthold) with an injector system, as described before (22 (link)). Briefly, 50 µL cell culture supernatant was placed into a 96-well opaque white plate (Nunc) and was mixed with 50 µL of PBS containing 1 µg/mL native coelenterazine (Sigma) prior to quantification.
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