Antibody-based Analysis of Ion Transporters

Antibodies anti‐NKCC2 were from Merck Millipore (AB3562P, Merck Millipore, Burlington, MA). The antibody against the phosphorylated threonine 96 and 101 NKCC2
¹⁹ (link) was kindly provided by Prof. Biff Forbush, Yale University. Antibodies anti‐NCC were from StressMarq Biosciences Inc. (SPC‐402D, StressMarq Biosciences Inc., Victoria, BC, Canada). Antibodies against the phosphorylated Thr 53 NCC were from Phosphosolutions (p1311‐53, Phosphosolutions, Aurora, CO). The antibody against human AQP2 was previously described.
²⁰ (link) Antibodies against the phosphorylated 256 AQP2 were from Abcam (ab111346, Abcam, Cambridge, UK). BRL37344 was from Santa Cruz Biotechnology (sc‐200154, Santa Cruz Biotechnology, Dallas, TX).

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Milano S., Saponara I., Gerbino A., Carmosino M., Svelto M, & Procino G. (2024). The β3‐AR agonist BRL37344 ameliorates the main symptoms of X‐linked nephrogenic diabetes insipidus in the mouse model of the disease. Journal of Cellular and Molecular Medicine, 28(8), e18301.

Publication 2024

AB3562P SPC‐402D ab111346

Corresponding Organization : University of Basilicata

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Variable analysis

independent variables

Antibodies anti‐NKCC2 from Merck Millipore
Antibody against the phosphorylated threonine 96 and 101 NKCC2 provided by Prof. Biff Forbush, Yale University
Antibodies anti‐NCC from StressMarq Biosciences Inc.
Antibodies against the phosphorylated Thr 53 NCC from Phosphosolutions
Antibody against human AQP2 previously described
Antibodies against the phosphorylated 256 AQP2 from Abcam
BRL37344 from Santa Cruz Biotechnology

dependent variables

Not explicitly mentioned

control variables

Not explicitly mentioned

controls

Positive control: Not specified
Negative control: Not specified

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