All 4T1 experiments were performed in 8- to10-week-old female BALB/c mice. CD45.2+ WT mice were purchased from Charles River Laboratories. TSLP-KO47 (link), and SPC-TSLP40 (link) mice were all backcrossed to BALB/c background for more than 10 generations. MTAG in C57BL/6 background mice were provided by S. Abrams (Roswell Park Cancer Institute). MTAG/TSLP-KO mice were generated from crossing MTAG mice to TSLP-KO mice in C57BL/6 background mice. All experiments were performed as approved by Benaroya Research Institute animal facility IACUC and in compliance with all relevant animal use guidelines and ethical regulations. All mice were bred and housed in specific-pathogen free conditions in Benaroya Research Institute animal facility.
The cell lines 4T1 (ATCC® CRL-2539™), MDA-MB-468 (ATCC® HTB-132™), and MCF 10A (ATCC® CRL-10317™) were all purchased from ATCC and cultured according to ATCC guidelines. AT3 cell line was generous provided by S. Abrams (Roswell Park Cancer Institute).
The cell lines 4T1 (ATCC® CRL-2539™), MDA-MB-468 (ATCC® HTB-132™), and MCF 10A (ATCC® CRL-10317™) were all purchased from ATCC and cultured according to ATCC guidelines. AT3 cell line was generous provided by S. Abrams (Roswell Park Cancer Institute).
