For mCherry in vivo fluorescence microscopy, yeast transformants were cultured as usual and cells were collected by centrifugation of 1 mL of culture at 2500 rpm. For actin staining, cells were cultured as usual, fixed with p-formaldehyde, and stained with rhodamine–phalloidin as described previously [39 (link)]. Samples were prepared for visualization and the microscope used was an Eclipse TE2000U (Nikon, Tokyo, Japan) with the appropriate sets of filters. Digital images were acquired with an Orca C4742-95-12ER charge-coupled device camera (Hamamatsu, Hamamatsu city, Japan) and processed with HCImage software (Hamamatsu).
Free full text: Click here
