Culturing Glioma Cell Lines

Human glioblastoma-astrocytoma U-87-MG (NCI-PBCF-HTB14; ATCC HTB-14) and human brain malignant glioma GBM 8401 cells were obtained from Bioresource Collection and Research Center (BCRC, Hsinchu, Taiwan) [21 (link)]. All cell lines were incubated in an atmosphere containing 5% CO₂ at 37°C. GBM8401 cells were cultured in a RPMI1640 medium with supplemental 10% fetal bovine serum (FBS) and U87 MG cells in modified Eagle's Medium (MEM) with supplemental 10% FBS.

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Tsai T.H., Lieu A.S., Wang Y.W., Yang S.F., Hsu Y.C, & Lin C.L. (2021). Therapeutic Potential of RTA 404 in Human Brain Malignant Glioma Cell Lines via Cell Cycle Arrest via p21/AKT Signaling. BioMed Research International, 2021, 5552226.

Publication 2021

GBM 8401

Astrocytoma Atmosphere Brain Cell lines Cells Fetal bovine serum Glioblastoma Human Malignant glioma

Corresponding Organization : I-Shou University

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Variable analysis

independent variables

Cell line (U-87-MG, GBM 8401)

dependent variables

Not explicitly mentioned

control variables

Atmosphere (5% CO2, 37°C)
Culture medium (RPMI1640 with 10% FBS for GBM8401, MEM with 10% FBS for U-87-MG)

controls

Positive control: Not specified
Negative control: Not specified

Annotations

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