As described in Delage et al. [19 (link)], HPLC analyses were done using an Ultimate 3000 SD System (Thermo Fisher Scientific, Waltham, MA, USA) and a GabiStar radiodetector (Elysia-Raytest GmBH, Straubenhard, Germany). A size exclusion chromatography was performed using phosphate buffer pH 6.8 as solvent and a 200 kDa size exclusion column (XBridge protein BEH, Waters, Baden-Dättwil, Switzerland). Each chromatography profile was analyzed at 280 nm.
Delage J.A., Faivre-Chauvet A., Barbet J., Fierle J.K., Schaefer N., Coukos G., Viertl D., Dunn S.M., Gnesin S, & Prior J.O. (2021). Impact of DOTA Conjugation on Pharmacokinetics and Immunoreactivity of [177Lu]Lu-1C1m-Fc, an Anti TEM-1 Fusion Protein Antibody in a TEM-1 Positive Tumor Mouse Model. Pharmaceutics, 13(1), 96.
BufferChromatography Hplc Phosphate Protein Size exclusion chromatography Solvent
Corresponding Organization : University of Lausanne
Other organizations :
Nantes Université, Université d'Angers, Centre National de la Recherche Scientifique, Inserm, Groupement d'Intérêt Economique, Ludwig Cancer Research
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