The MAGL inhibitor JZL184 (40 mg/kg) and R-flurbiprofen (9 mg/kg) were purchased from Cayman Chemicals, Ann Arbor, MI; Lumiracoxib (5 mg/kg) from Selleck Chemicals, TX, USA; and LM-4131 (10 mg/kg), was synthesized as previously described29 (link); and were administered via intraperitoneal injection (1 ml/kg DMSO) 3 h before sacrifice. LPS (Sigma-Aldrich, St. Louis, MO), was administered via intraperitoneal injection (10 ml/kg; pyrogen-free saline), with two injections of LPS given, 24 h apart. Four hours after the second injection, mice were treated with JZL184 (40 mg/kg). Mice were sacrificed 3 hours after JZL184 or DMSO injection.
Protocol detail
Inhibition of Neuroinflammation Pathways
Partial Protocol Preview
This section provides a glimpse into the protocol.
The remaining content is hidden due to licensing restrictions, but the full text is available at the following link:
Access Free Full Text.
Cayman
Dmso
Intraperitoneal injection
Jzl184
Lm 4131
Lumiracoxib
Mice
Pyrogen
R flurbiprofen
Saline
Corresponding Organization : Vanderbilt University Medical Center
Top 5 similar protocols
Variable analysis
independent variables
- JZL184 (40 mg/kg)
- R-flurbiprofen (9 mg/kg)
- Lumiracoxib (5 mg/kg)
- LM-4131 (10 mg/kg)
- LPS (10 ml/kg; pyrogen-free saline)
- Not explicitly mentioned
- Time of administration: 3 h before sacrifice for JZL184, R-flurbiprofen, Lumiracoxib, and LM-4131; two injections of LPS 24 h apart, with JZL184 administered 4 h after the second LPS injection
- Route of administration: Intraperitoneal injection
- Solvent: DMSO (1 ml/kg) for JZL184, R-flurbiprofen, Lumiracoxib, and LM-4131; pyrogen-free saline for LPS
- Not explicitly mentioned
- Not explicitly mentioned
Annotations
Based on most similar protocols
Etiam vel ipsum. Morbi facilisis vestibulum nisl. Praesent cursus laoreet felis. Integer adipiscing pretium orci. Nulla facilisi. Quisque posuere bibendum purus. Nulla quam mauris, cursus eget, convallis ac, molestie non, enim. Aliquam congue. Quisque sagittis nonummy sapien. Proin molestie sem vitae urna. Maecenas lorem.
Sign up for free or login to display all annotations
As authors may omit details in methods from publication, our AI will look for missing critical information across the 5 most similar protocols.
About PubCompare
Our mission is to provide scientists with the largest repository of trustworthy protocols and intelligent analytical tools, thereby offering them extensive information to design robust protocols aimed at minimizing the risk of failures.
We believe that the most crucial aspect is to grant scientists access to a wide range of reliable sources and new useful tools that surpass human capabilities.
However, we trust in allowing scientists to determine how to construct their own protocols based on this information, as they are the experts in their field.
Ready to get started?
Sign up for free.
Registration takes 20 seconds.
Available from any computer
No download required
Revolutionizing how scientists
search and build protocols!