HPLC Analysis of Panax Ginseng, Coptis Chinensis, and Ligusticum Chuanxiong in YQF Powder

The reference standards or YQF powder were precisely weighed and dissolved with methanol solution. Inertsil C18 (150 mm × 4.6 mm, 5 μm) was used as the stationary phase for the chromatographic separation. The compounds were identified by individual peak retention times compared to reference substances. The detection wavelength of the main components of Panax ginseng C.A.Mey (Renshen) in YQF was 203 nm, with a column temperature at 35°C. The flow rate was 1 ml/min and the total injection volume was 20 μL. The mobile phase consisted of solvent A (acetonitrile) and solvent B (water) with the following gradient elution: 18% A at 0–40 min; 21% A at 40–42 min; 26% A at 42–46 min; 32% A at 46–66 min; 33.5% A at 66–71 min; 38% A at 71–86 min; 65% A at 86–96 min; 85% A at 96–103 min (Guo, 2014 ). The detection wavelength of the main components of Coptis chinensis Franch (Huanglian) in YQF was 345 nm, with a column temperature at 25°C. The flow rate was 1 mL/min, and the total injection volume was 5 μL. The mobile phase consisted of 70% solvent A (0.05% trifluoroacetic acid) and 30% solvent B (acetonitrile). The detection wavelength of the main components of Ligusticum chuanxiong S.H.Qiu, Y.Q.Zeng, K.Y.Pan, Y.C.Tang and J.M.Xu (Chuanxiong) in YQF was 294 nm, with a column temperature at 30°C. The flow rate was 1 mL/min, and the total injection volume was 5 μL. The mobile phase consisted of 60% solvent A (0.05% trifluoroacetic acid) and 40% solvent B (methanol).