Sections were proceeded to histomorphological examinations after laser confocal imaging. Goldner's trichrome staining protocol was used to stain the sections [23 ].
Microscope images were obtained using the Eclipse LV100 POL (Nikon, Japan). Areas of osteonecrosis were defined by 8-10 adjacent empty osteocytic lacunae with the loss of osteocytes [24 (link), 25 (link)]. Evaluation of osteonecrosis was determined as either no osteonecrosis or osteonecrosis presenting in the ten randomly selected high-power fields (20× ), the incidence of which was calculated by dividing the number of animals with osteonecrosis by the total number of animals in a group at each time point.
Microscope images were obtained using the Eclipse LV100 POL (Nikon, Japan). Areas of osteonecrosis were defined by 8-10 adjacent empty osteocytic lacunae with the loss of osteocytes [24 (link), 25 (link)]. Evaluation of osteonecrosis was determined as either no osteonecrosis or osteonecrosis presenting in the ten randomly selected high-power fields (20
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