Identification and Characterization of Staphylococcus aureus
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Corresponding Organization : King Abdulaziz University
Other organizations : Jeddah University, Al Jouf University, Beni-Suef University, Al-Azhar University
Variable analysis
- Aseptic dry swab samples taken from the pus and wounds
- Identification of Staphylococcal isolates using biochemical and morphological approaches
- Identification of S. aureus based on the presence of catalase, oxidase, coagulase activity, and DNase activity
- Confirmation of presumptive MRSA using Vitek 2 identification card
- Evaluation of methicillin susceptibility using oxacillin discs and identification of the mecA gene using PCR
- Proper labeling of the samples before being transferred to the lab
- Prompt processing of the specimens in the lab
- Culturing the specimens on Blood Agar and incubating at 37°C for 24 hours
- Using the QC strain S. aureus ATCC25923 with each run of the Vitek 2 identification
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