This assay was implemented as previously described [24 (link)]. TOP/FOP-Flash (Genechem) was co-transfected into CC cells with pcDNA3.1-PDHB-AS or empty vector (pcDNA3.1). After transfection for 48 h, both firefly and Renila luciferase activities were measured. The luciferase activity of TOP/FOP-Flash was normalized to the Renilla luciferase activity. Experiments were performed in triplicate.
Free full text: Click here