Inducing M2 Macrophages with Chitin

Female C57BL/6 mice were injected intraperitoneally with 50 µg of chitin particles from shrimp shells (C9752, Sigma-Aldrich) for 3 hr to induce M2 macrophages, followed by the first dosage of GW4064 (30 mg/kg) and 2 mg/kg LE540 or vehicle control. A second dose of GW4064 and LE540 at the same concentration as the first was then administered and the peritoneal macrophages collected for analysis.

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Jaroonwitchawan T., Arimochi H., Sasaki Y., Ishifune C., Kondo H., Otsuka K., Tsukumo S.I, & Yasutomo K. (2023). Stimulation of the farnesoid X receptor promotes M2 macrophage polarization. Frontiers in Immunology, 14, 1065790.

Publication 2023

C9752

C57bl mice Chitin Female Gw4064 Le540 Macrophages Peritoneal macrophages

Corresponding Organization : Tokushima University

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Variable analysis

independent variables

Injection of 50 µg of chitin particles from shrimp shells (C9752, Sigma-Aldrich) for 3 hr
Administration of GW4064 (30 mg/kg) and LE540 (2 mg/kg) or vehicle control

dependent variables

Peritoneal macrophages collected for analysis

control variables

Injection volume
Time period of 3 hr for chitin particle injection
Concentration of GW4064 (30 mg/kg) and LE540 (2 mg/kg)

controls

Vehicle control for GW4064 and LE540

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