Optical Assessment of Cardiomyocyte Shortening

Unloaded CM shortening was assessed optically, as described previously [14 (link)]. Briefly, contractions were triggered by field stimulation, as described above, at room temperature. CM were imaged using an inverted microscope with a 40× air objective using phase contrast (Leica DM IRBE microscope; Leica Microsystems, Vienna, Austria). Sarcomere shortening dynamics were quantified using the IonWizard 6.6 software package (IonOptix, Westwood, MA, USA). CM with a resting sarcomere length (SL) shorter than 1.65 µm were excluded from the analyses.

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Greiner J., Schiatti T., Kaltenbacher W., Dente M., Semenjakin A., Kok T., Fiegle D.J., Seidel T., Ravens U., Kohl P., Peyronnet R, & Rog-Zielinska E.A. (2022). Consecutive-Day Ventricular and Atrial Cardiomyocyte Isolations from the Same Heart: Shifting the Cost–Benefit Balance of Cardiac Primary Cell Research. Cells, 11(2), 233.

Publication 2022

Leica DM IRBE microscope IonWizard 6.6

Microscope Phase contrast Sarcomere

Corresponding Organization : University of Freiburg

Other organizations : University of Florence, Friedrich-Alexander-Universität Erlangen-Nürnberg

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Variable analysis

independent variables

Field stimulation to trigger contractions

dependent variables

Unloaded CM shortening
Sarcomere shortening dynamics

control variables

Room temperature
Resting sarcomere length (SL) longer than 1.65 µm

controls

Positive control: Not explicitly mentioned.
Negative control: Not explicitly mentioned.

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