Caspase Activation in Ovarian Cancer

Caspase 3/7, Caspase 8, and Caspase 9 activity were measured in OVCAR3 and OVCAR8 cells lines using Caspase-Glo luminescence assays (Promega, G8091, G8201, G8211) according to the manufacturer’s specifications after exposing cells to panobinostat 10 nM alone and in combination with birinapant 20 μM for 24 h. All drug exposures occurred with or without 10 ng/mL of TNF-α. Activity data were normalized to viable cell number and measured in an identical plate by XTT assay as described.

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Shibuya Y., Kudo K., Zeligs K.P., Anderson D., Hernandez L., Ning F., Cole C.B., Fergusson M., Kedei N., Lyons J., Taylor J., Korrapati S, & Annunziata C.M. (2023). SMAC Mimetics Synergistically Cooperate with HDAC Inhibitors Enhancing TNF-α Autocrine Signaling. Cancers, 15(4), 1315.

Publication 2023

G8091 G8201 G8211

Assay Birinapant Caspase Caspase 7 Caspase 8 Caspase 9 Cells Cells lines Drug Luminescence assays Panobinostat Promega Tnf α

Corresponding Organization : National Institutes of Health

Other organizations : Tohoku University, Walter Reed National Military Medical Center, Icahn School of Medicine at Mount Sinai

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Variable analysis

independent variables

Panobinostat 10 nM
Birinapant 20 μM
TNF-α 10 ng/mL

dependent variables

Caspase 3/7 activity
Caspase 8 activity
Caspase 9 activity
Viable cell number

control variables

OVCAR3 and OVCAR8 cell lines
Caspase-Glo luminescence assays (Promega, G8091, G8201, G8211)
XTT assay for viable cell number
Exposure duration of 24 h

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