Assessing Apoptosis in A549 Cells

The A549 cells in the logarithmic growth phase were treated with 125, 250, and 500 μg/mL LLTPs for 48 h. Then, the cells were washed with phosphate-buffered saline (PBS) three times, suspended in annexin V–FITC binding solution, mixed with annexin V–FITC and propidium iodide staining solution (Thermo Fisher Scientific, Inc.), and cultured at 4°C in the dark for 15 min. Finally, the apoptosis of cancer cells was analyzed by flow cytometry (FACSCalibur, BD, NJ, USA) [15 (link)].

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Chen J., Du Y., Long Y., Tao D., Hu M., Jiang Y., Wan Y, & Yang D. (2022). Polyphenols in Ilex latifolia Thunb. inhibit human lung cancer cell line A549 by regulation of the PI3K-Akt signaling pathway. BMC Complementary Medicine and Therapies, 22, 85.

Publication 2022

annexin V–FITC binding solution annexin V–FITC and propidium iodide staining solution FACSCalibur

A549 cells Annexin v fitc Apoptosis Cancer Flow cytometry Phosphate Propidium iodide Saline

Corresponding Organization :

Other organizations : Chongqing Cancer Hospital, Chongqing University

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Variable analysis

independent variables

LLTP concentrations (125, 250, and 500 μg/mL)

dependent variables

Apoptosis of cancer cells

control variables

Annexin V–FITC binding solution
Propidium iodide staining solution
Culture conditions (4°C in the dark for 15 min)

controls

No positive control was explicitly mentioned.
No negative control was explicitly mentioned.

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