MSC-seeded scaffolds and meniscus repair model inner cores were
digested in 0.5 mL or 1.5 mL, respectively, of 125 μg/mL papain
overnight at 65°C54 (link),
67 , 68 (link). DNA content was measured using
the picogreen assay (Invitrogen, Carlsbad, CA). Sulfated
glycosaminoglycan (sGAG) content was measured using the
dimethylmethylene blue assay69 (link) and bovine chondroitin sulfate as a standard
(Sigma-Aldrich). Collagen content was assessed with the hydroxyproline
(OHP) assay70 (link), using
trans-4-hydroxy-L-proline (Sigma-Aldrich) as a standard, as previously
described54 (link), 67 , 68 (link).
digested in 0.5 mL or 1.5 mL, respectively, of 125 μg/mL papain
overnight at 65°C54 (link),
67 , 68 (link). DNA content was measured using
the picogreen assay (Invitrogen, Carlsbad, CA). Sulfated
glycosaminoglycan (sGAG) content was measured using the
dimethylmethylene blue assay69 (link) and bovine chondroitin sulfate as a standard
(Sigma-Aldrich). Collagen content was assessed with the hydroxyproline
(OHP) assay70 (link), using
trans-4-hydroxy-L-proline (Sigma-Aldrich) as a standard, as previously
described54 (link), 67 , 68 (link).
