Collagenase-Induced Intracerebral Hemorrhage in Mice
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Corresponding Organization :
Other organizations : University of Georgia, Washington University in St. Louis
Protocol cited in 1 other protocol
Variable analysis
- Intracerebral injection of collagenase
- Outcomes measured on 2, 5, and 10 days post injury (dpi) after transcardial perfusion and brain sectioning
- Intraperitoneal injection of avertin (500 mg/kg of body weight) for anesthesia
- Mice placed in a stereotaxic frame
- 24-gauge needle inserted through a burr hole into the striatum at specific coordinates
- Collagenase (type VII-S; 0.225U in 0.5 μl saline) administered over 5 minutes
- Needle kept in place for 10 minutes to prevent reflux
- Positive control: Not explicitly mentioned
- Negative control: Not explicitly mentioned
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