Synaptophysin-Tagged GCaMP6s Expression

GCaMP6s21 (link) was fused in-frame to synaptophysin22 (link) and ligated into the Cre-dependent pAAV-hSyn-DIO-WPRE backbone23 (link). A Cre-independent version of this expression plasmid was generated by treatment of the DIO vector with Cre recombinase (NEB) in vitro.

Partial Protocol Preview
This section provides a glimpse into the protocol.
The remaining content is hidden due to licensing restrictions, but the full text is available at the following link: Access Free Full Text.

Mahn M., Prigge M., Ron S., Levy R, & Yizhar O. (2016). Biophysical constraints of optogenetic inhibition at presynaptic terminals. Nature neuroscience, 19(4), 554-556.

Publication 2016

Cre recombinase

Cre recombinase Frame Plasmid Vector

Corresponding Organization :

Other organizations : Weizmann Institute of Science

Top 5 similar protocols

Variable analysis

independent variables

Treatment of the DIO vector with Cre recombinase (NEB) in vitro

dependent variables

Expression of the GCaMP6s-synaptophysin fusion protein

control variables

Cre-dependent pAAV-hSyn-DIO-WPRE backbone

controls

Positive control: Cre-dependent expression of the GCaMP6s-synaptophysin fusion protein
Negative control: Cre-independent expression of the GCaMP6s-synaptophysin fusion protein

Annotations

Based on most similar protocols

Etiam vel ipsum. Morbi facilisis vestibulum nisl. Praesent cursus laoreet felis. Integer adipiscing pretium orci. Nulla facilisi. Quisque posuere bibendum purus. Nulla quam mauris, cursus eget, convallis ac, molestie non, enim. Aliquam congue. Quisque sagittis nonummy sapien. Proin molestie sem vitae urna. Maecenas lorem.

As authors may omit details in methods from publication, our AI will look for missing critical information across the 5 most similar protocols.

About PubCompare

Our mission is to provide scientists with the largest repository of trustworthy protocols and intelligent analytical tools, thereby offering them extensive information to design robust protocols aimed at minimizing the risk of failures.

We believe that the most crucial aspect is to grant scientists access to a wide range of reliable sources and new useful tools that surpass human capabilities.

However, we trust in allowing scientists to determine how to construct their own protocols based on this information, as they are the experts in their field.

Ready to get started?

Revolutionizing how scientists
search and build protocols!