Zika Virus Infection in Mice

Three-day-old Swiss mice were infected intraperitoneally with 2 × 10⁴ PFU of the Brazilian ZIKV strain. Treatments with iDGAT-1 were carried out at 2.5 mg/kg/day intraperitoneally and started one day before infection (pretreatment). The same dose was given once daily for the subsequent days throughout the experiment. For comparisons, a mock-infected group of animals was used as a control. Animals were monitored daily for survival and weight gain; euthanasia was performed to alleviate animal suffering when necessary. The criteria for humane endpoint were differences in weight between infected and control groups > 25%, ataxia or rejection of moribund offspring, characterized by no feeding by the female adult mouse.

Free full text: Click here

Dias S.S., Cunha-Fernandes T., Souza-Moreira L., Soares V.C., Lima G.B., Azevedo-Quintanilha I.G., Santos J., Pereira-Dutra F., Freitas C., Reis P.A., Rehen S.K., Bozza F.A., Souza T.M., de Almeida C.J, & Bozza P.T. (2023). Metabolic reprogramming and lipid droplets are involved in Zika virus replication in neural cells. Journal of Neuroinflammation, 20, 61.

Publication 2023

Adult Animal Ataxia Euthanasia Female Infection Mouse Strain Swiss mice Zikv

Corresponding Organization :

Other organizations : Fundação Oswaldo Cruz, Universidade do Estado do Rio de Janeiro, D’Or Institute for Research and Education

Top 5 similar protocols

Variable analysis

independent variables

Treatment with iDGAT-1 at 2.5 mg/kg/day intraperitoneally
Timing of iDGAT-1 treatment (started one day before infection)

dependent variables

Survival
Weight gain

control variables

Age of mice (three-day-old)
Mouse strain (Swiss)
Infection method (intraperitoneally)
ZIKV strain (Brazilian)
ZIKV dose (2 × 10^4 PFU)

controls

Positive control: Mock-infected group of animals

Annotations

Based on most similar protocols

Etiam vel ipsum. Morbi facilisis vestibulum nisl. Praesent cursus laoreet felis. Integer adipiscing pretium orci. Nulla facilisi. Quisque posuere bibendum purus. Nulla quam mauris, cursus eget, convallis ac, molestie non, enim. Aliquam congue. Quisque sagittis nonummy sapien. Proin molestie sem vitae urna. Maecenas lorem.

As authors may omit details in methods from publication, our AI will look for missing critical information across the 5 most similar protocols.

About PubCompare

Our mission is to provide scientists with the largest repository of trustworthy protocols and intelligent analytical tools, thereby offering them extensive information to design robust protocols aimed at minimizing the risk of failures.

We believe that the most crucial aspect is to grant scientists access to a wide range of reliable sources and new useful tools that surpass human capabilities.

However, we trust in allowing scientists to determine how to construct their own protocols based on this information, as they are the experts in their field.

Ready to get started?

Revolutionizing how scientists
search and build protocols!