Pancreatic Cancer and Lung Adenocarcinoma Cell Lines

Pancreatic cancer cell lines (T4- and T3-KPC cells) were derived from KPC mice as previously described (43 (link)) and were a gift from David Tuveson (Cold Spring Harbor Laboratory, Long Island, New York, USA). MS1, a mouse endothelial pancreas cell line, and CFPAC were obtained from ATCC. The Kras^LA1/+p53^R172H/Δg/+ lung adenocarcinoma cell line (393P) was generated as previously described (44 (link)). All cell lines were cultured in DMEM (Thermo Fisher Scientific) with 10% FBS, 100 IU/mL penicillin, and 100 μg/mL streptomycin, and incubated at 37°C in a humidified incubator with 5% CO₂. C2C12 myoblasts were purchased from ATCC and cultured in DMEM with 10% FBS until confluent. After reaching confluency, the myoblasts were differentiated in DMEM with 2% horse serum and 1 μg/mL insulin for 72 hours, as previously described (22 (link)). 3-MA (item no. 13242) for in vitro and in vivo studies was purchased from Cayman Chemical.