Artificial Sandfly Infection Protocol

For artificial infection of sandflies, defibrinated rabbit blood was used. Red blood cells were centrifuged and washed in sterile PBS 3 times and serum was inactivated at 56 °C for 30 to 45 min. The parasites were collected on early log phase culture, resuspended in the inactivated serum, and later mixed with the red blood cells. Artificial infections were performed with blood containing 10⁷ parasites/mL of blood using a Hemotek artificial feeder.

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Tano F.T., Telleria E.L., Rêgo F.D., Coelho F.S., de Rezende E., Soares R.P., Traub-Cseko Y.M, & Stolf B.S. (2023). Phenotypical Differences between Leishmania (Leishmania) amazonensis PH8 and LV79 Strains May Impact Survival in Mammal Host and in Phlebotomine Sand Flies. Pathogens, 12(2), 173.

Publication 2023

artificial feeder

Blood Infections Parasites Rabbit Red blood cells Sandflies Serum Sterile

Corresponding Organization : Universidade de São Paulo

Other organizations : Fundação Oswaldo Cruz, Charles University

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Variable analysis

independent variables

Blood containing 10^7 parasites/mL

dependent variables

Artificial infection of sandflies

control variables

Defibrinated rabbit blood used
Red blood cells centrifuged and washed in sterile PBS 3 times
Serum inactivated at 56°C for 30 to 45 min
Parasites collected on early log phase culture, resuspended in the inactivated serum, and later mixed with the red blood cells
Artificial infections performed using a Hemotek artificial feeder

controls

Positive control: Not explicitly mentioned.
Negative control: Not explicitly mentioned.

Annotations

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