According to user instructions, autophagy-detecting dyes (Dojindo, Kumamoto, Japan), such as 12.5 nM DAPGreen [35 (link)] and 125 nM DALGreen [36 (link)], were chosen to probe autophagosomes/autolysosomes and autolysosomes, respectively. After PBS washing, drug-treated cells were detected and quantified by flow cytometry.
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