Culturing Endometrial Cancer Cell Lines

HEC1B and KLE type II human endometrial cancer cell lines were purchased from the American Type Culture Collection (Manassas, VA) [61 (link)]. HEC-1B cells were cultured in Minimal Essential Medium (Gibco, Life Technologies, Burlington, ON) supplemented with 10% fetal bovine serum (FBS; Hyclone Laboratories Inc., Logan, UT). KLE cells were cultured in Dulbecco's Modified Eagle Medium/Nutrient Mixture F-12 (Gibco, Life Technologies) supplemented with 10% fetal bovine serum (FBS; Hyclone Laboratories Inc.). Cultures were maintained at 37°C in a humidified atmosphere of 5% CO2 in air.

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Xiong S., Klausen C., Cheng J.C., Zhu H, & Leung P.C. (2015). Activin B induces human endometrial cancer cell adhesion, migration and invasion by up-regulating integrin β3 via SMAD2/3 signaling. Oncotarget, 6(31), 31659-31673.

Publication 2015

HEC1B Minimal Essential Medium Dulbecco's Modified Eagle Medium/Nutrient Mixture F-12 fetal bovine serum

Atmosphere Cell lines Cells Cultured medium Eagle Endometrial cancer Human Nutrient

Corresponding Organization : Child and Family Research Institute

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Variable analysis

independent variables

Cell lines: HEC1B and KLE type II human endometrial cancer cell lines

dependent variables

Not explicitly mentioned

control variables

Culture conditions: Cultures were maintained at 37°C in a humidified atmosphere of 5% CO2 in air
Culture media: HEC-1B cells were cultured in Minimal Essential Medium (Gibco, Life Technologies, Burlington, ON) supplemented with 10% fetal bovine serum (FBS; Hyclone Laboratories Inc., Logan, UT), and KLE cells were cultured in Dulbecco's Modified Eagle Medium/Nutrient Mixture F-12 (Gibco, Life Technologies) supplemented with 10% fetal bovine serum (FBS; Hyclone Laboratories Inc.)

controls

Positive control: None mentioned
Negative control: None mentioned

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