STBEV Internalization in Endothelial Cells

For in vitro STBEV-exposure experiments, prepared vessel segments were immersed in carbonated Na-Krebs buffer (pH 7.3–7.4) in Eppendorf tubes, while gently shaking. Vessels were incubated for 2 h at 37 °C with buffer, or buffer containing 40 µg/ml PE or normal STBEVs with and without 10 µg/ml chlorpromazine (Sigma-Aldrich) or 10 µg/ml anti-LOX-1 antibody (TS92, provided by Professor Tatsuya Sawamura, Japan). The vessels were thereafter fixed for further microscopy analysis. The experiment was run twice. As stated above, we have previously shown that placenta-derived STBEVs are internalized by human endothelial cells within 30 min of incubation^{32 (link)}.