The MC crude extract was dissolved with deionized water, and the concentration of paeonol was 0.8 mg/mL. A quantity (1.0 g) of the different types of macroporous resins (LSA-900C, HPD-100, HPD-300, AB-8, S-8, D-101, NKA-9, HPD-826) that had been pre-treated were mixed with 25 mL MC crude extract (0.8 mg/mL) in 50 mL conical flasks. All the flasks were put in a constant temperature shaker (120 rpm) and shaken for 24 h at 25 °C. Following the absorption equilibrium, the resins were filtered, and the content of paeonol in the solutions was determined by HPLC after adsorption equilibrium.
After adsorption saturation, the different types of macroporous resins were washed with distilled water until the surface of resin had no residual MC extract, and the surface water of the macroporous resins was sucked up with filter paper. Subsequently, the resins were desorbed with 25 mL of 50% aqueous ethanol solution in a constant temperature water bath shaker (120 rpm) for 24 h at 25 °C. After desorption, the concentration of paeonol was measured. The adsorption capacity, adsorption ratio, desorption ratio, and recovery ratio were calculated according to the following Equations (2)–(5):
Adsorption capacity (mg/g)
Adsorption ratio (%)
Desorption ratio (%)
Recovery ratio (%)
where Qe (mg/g) is the adsorption capacity at adsorption equilibrium; C0 and C1 (mg/mL) are the initial and equilibrium concentrations of paeonol in the solutions, respectively; V0 (mL) is the volume of the crude extract; M (g) is the weight of the resin; V1 (mL) is the volume of the crude extract after adsorption; C2 (mg/mL) is the concentration of paeonol in the desorption solution; V2 (mL) is the volume of the desorption solution; and R (%) is the recovery ratio.
After adsorption saturation, the different types of macroporous resins were washed with distilled water until the surface of resin had no residual MC extract, and the surface water of the macroporous resins was sucked up with filter paper. Subsequently, the resins were desorbed with 25 mL of 50% aqueous ethanol solution in a constant temperature water bath shaker (120 rpm) for 24 h at 25 °C. After desorption, the concentration of paeonol was measured. The adsorption capacity, adsorption ratio, desorption ratio, and recovery ratio were calculated according to the following Equations (2)–(5):
Adsorption capacity (mg/g)
Adsorption ratio (%)
Desorption ratio (%)
Recovery ratio (%)
where Qe (mg/g) is the adsorption capacity at adsorption equilibrium; C0 and C1 (mg/mL) are the initial and equilibrium concentrations of paeonol in the solutions, respectively; V0 (mL) is the volume of the crude extract; M (g) is the weight of the resin; V1 (mL) is the volume of the crude extract after adsorption; C2 (mg/mL) is the concentration of paeonol in the desorption solution; V2 (mL) is the volume of the desorption solution; and R (%) is the recovery ratio.
Free full text: Click here
