NFE2L3 Modulation in Gastric Cancer

Plasmids (pcDNA3.1) were purchased from Shanghai Genechem. Plasmids were transfected into AGS via Lipofectamine 2000 (ThermoFisher). siRNA (siNFE2L3#1, siNFE2L3#2, siNFE2L3#3) purchased from RIBOBIO and transfected into HGC-27 using Lipofectamine 2000. qRT-PCR was applied to detect the efficiency of NFE2L3 overexpression or knockdown.
The sequences of siRNA were as follows:
siNFE2L3#1 GCATGACCTTTATCATGAT
siNFE2L3#2 GTATGACCTTGACATAAAT
siNFE2L3#3 GTCAGGATGTGAATCTTCA

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Li F, & Wen Z. (2024). Identification roles of NFE2L3 in digestive system cancers. Journal of Cancer Research and Clinical Oncology, 150(3), 150.

Publication 2024

pcDNA3.1 Lipofectamine 2000

Corresponding Organization :

Other organizations : Nanchang University, Second Affiliated Hospital of Nanchang University

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Variable analysis

independent variables

Overexpression of NFE2L3 using pcDNA3.1 plasmid
Knockdown of NFE2L3 using siRNA (siNFE2L3#1, siNFE2L3#2, siNFE2L3#3)

dependent variables

Efficiency of NFE2L3 overexpression or knockdown as measured by qRT-PCR

control variables

Lipofectamine 2000 used for transfection of plasmids and siRNA
AGS cell line used for plasmid transfection
HGC-27 cell line used for siRNA transfection

controls

No positive control specified
No negative control specified

Annotations

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