Flow cytometry cell surface staining

Cell surface staining for flow cytometry was performed using the following antibodies: CD3-AF700 (clone UCHT1, BD), CD4-APC-H7 (clone RPA-T4, BD), CD8-APC-H7 (clone SK1, BD) and PD-1-BV421 (clone EH12.2H7, Biolegend). Isotype-matched antibodies, labeled with the proper fluorochromes, were used as negative controls. Cells were analyzed using a BD Fortessa flow cytometer (BD Biosciences), and data analysis was performed with Flowjo 10.6 software as previously described (22 (link)).

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Geng S., Xu R., Huang X., Li M., Deng C., Lai P., Wang Y., Wu P., Chen X., Weng J, & Du X. (2022). Dynamics of PD-1 expression are associated with treatment efficacy and prognosis in patients with intermediate/high-risk myelodysplastic syndromes under hypomethylating treatment. Frontiers in Immunology, 13, 950134.

Publication 2022

CD3-AF700 (clone UCHT1, CD4-APC-H7 (clone RPA-T4, CD8-APC-H7 (clone SK1, PD-1-BV421 (clone EH12.2H7, BD Fortessa flow cytometer

Antibodies Cells Clone Flow cytometry Fluorochromes Isotype

Corresponding Organization : Guangdong Provincial People's Hospital

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Variable analysis

independent variables

CD3-AF700 (clone UCHT1, BD)
CD4-APC-H7 (clone RPA-T4, BD)
CD8-APC-H7 (clone SK1, BD)
PD-1-BV421 (clone EH12.2H7, Biolegend)

dependent variables

Not explicitly mentioned

control variables

Isotype-matched antibodies, labeled with the proper fluorochromes, were used as negative controls.

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