Resazurin Reduction Assay for Cell Viability

The resazurin reduction assay to determine cell viability and metabolism was performed according to Ivanov et al. (2014) (link) with modifications. In brief, a stock solution (600 µM in PBS) was aliquoted and stored at −18°C. On the day of analysis, a working solution of 60 µM resazurin in the DMEM/F12 medium was prepared and protected from light. After 24 h of treatment, the medium in the wells was replaced with a working solution of resazurin (100 µL), and the plates were again stored at 37°C. Absorbance was measured at 570 and 600 nm using a microplate reader (BioTek Instruments, United States) 30 min and 4 h after the addition of the dye.