Polygalacturonase (PG) activity was assessed usng the 3,5-dinitrosalicylic acid (DNS) reaction, as described by Miller28 (link), with polygalacturonic acid serving as the substrate. Calibration of the system was accomplished through a concentration curve of galacturonic acid ranging from 0.1 to 1.0 mg mL−1. An enzyme unit was defined as the quantity of enzyme required to liberate 1 µmol of galacturonic acid per minute under the specified test conditions.
Protein quantification followed the Bradford method employing bovine serum albumin as the standard29 (link).
Protein quantification followed the Bradford method employing bovine serum albumin as the standard29 (link).
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