Protocol detail

Western Blot Analysis of Cell Signaling

Find Similar Protocols

The primary antibodies used in this study were IG2BP2 (11601–1-AP; ProteinTech Group, Inc), Phospho-Akt (Ser473) (#4060, CST), GAPDH (#5174, CST), Akt (#4691, CST), cleaved PARP (#5625, CST), cleaved caspase-3 (#9664, CST), CDK2 (#2546, CST), p21 Waf1/Cip1 (#2947, CST) and Cyclin D1 (#2978, CST). The secondary antibodies and enhanced chemiluminescence reagent were purchased from Boster (Wuhan, China). The protein extraction and western blot analysis were performed as described in our previous study [5 (link)].

Free full text: Click here

Xu X., Yu Y., Zong K., Lv P, & Gu Y. (2019). Up-regulation of IGF2BP2 by multiple mechanisms in pancreatic cancer promotes cancer proliferation by activating the PI3K/Akt signaling pathway. Journal of Experimental & Clinical Cancer Research : CR, 38, 497.

Publication 2019

enhanced chemiluminescence reagent

Antibodies Caspase 3 Cdk2 Chemiluminescence Cyclin d1 Gapdh Protein Waf1 cip1 Western blot analysis

Top 5 similar protocols

Variable analysis

independent variables

IG2BP2 (11601–1-AP; ProteinTech Group, Inc)
Phospho-Akt (Ser473) (#4060, CST)
GAPDH (#5174, CST)
Akt (#4691, CST)
Cleaved PARP (#5625, CST)
Cleaved caspase-3 (#9664, CST)
CDK2 (#2546, CST)
P21 Waf1/Cip1 (#2947, CST)
Cyclin D1 (#2978, CST)

dependent variables

Protein expression levels

control variables

GAPDH (#5174, CST)

Annotations

Based on most similar protocols

Etiam vel ipsum. Morbi facilisis vestibulum nisl. Praesent cursus laoreet felis. Integer adipiscing pretium orci. Nulla facilisi. Quisque posuere bibendum purus. Nulla quam mauris, cursus eget, convallis ac, molestie non, enim. Aliquam congue. Quisque sagittis nonummy sapien. Proin molestie sem vitae urna. Maecenas lorem.

As authors may omit details in methods from publication, our AI will look for missing critical information across the 5 most similar protocols.

About PubCompare

Our mission is to provide scientists with the largest repository of trustworthy protocols and intelligent analytical tools, thereby offering them extensive information to design robust protocols aimed at minimizing the risk of failures.

We believe that the most crucial aspect is to grant scientists access to a wide range of reliable sources and new useful tools that surpass human capabilities.

However, we trust in allowing scientists to determine how to construct their own protocols based on this information, as they are the experts in their field.

Ready to get started?

Revolutionizing how scientists
search and build protocols!