Histological Evaluation of Lateral Condyle

The halves of the lateral condyles dedicated to histology were decalcified in formic acid/hydrochloric acid for about 1 month and then processed for paraffin embedding as indicated in a previous study [17 (link)]. Three slides of the central portion of the condyles were stained with Safranin O/Fast Green staining (Sigma-Aldrich, St. Louis, MO, USA) and the other three were immunostained for COLL II, using primary antibodies (anti-COLL II). Each slide was acquired with a Aperio ScanScope digital scanner (Aperio ScanScope CS, Aperio Technologies, Leica Biosystems, Milan, Italy). The three slides stained with Safranin O/Fast Green were histomorphometrically evaluated by measuring cartilage thickness (CT, µm) and fibrillation index (FI, %). In the three slides immunostained for COLL II content (%), the ratio between immunopositive stained areas and the total region of interest (ROI) areas was calculated.

Free full text: Click here

Berni M., Veronesi F., Fini M., Giavaresi G, & Marchiori G. (2023). Relations between Structure/Composition and Mechanics in Osteoarthritic Regenerated Articular Tissue: A Machine Learning Approach. International Journal of Molecular Sciences, 24(17), 13374.

Publication 2023

Safranin O/Fast Green staining Aperio ScanScope CS

Antibodies Cartilage Condyles Fast green Formic acid Hydrochloric acid Safranin o Scanner

Corresponding Organization : Istituto Ortopedico Rizzoli

Other organizations : Istituti di Ricovero e Cura a Carattere Scientifico

Top 5 similar protocols

Variable analysis

independent variables

Decalcification in formic acid/hydrochloric acid for about 1 month

dependent variables

Cartilage thickness (CT, µm)
Fibrillation index (FI, %)
Collagen II (COLL II) content (%)

control variables

Processing for paraffin embedding as indicated in a previous study [17]
Staining with Safranin O/Fast Green
Immunostaining for COLL II using primary antibodies (anti-COLL II)
Acquisition of slides using Aperio ScanScope digital scanner

controls

Positive control: Not specified
Negative control: Not specified

Annotations

Based on most similar protocols

Etiam vel ipsum. Morbi facilisis vestibulum nisl. Praesent cursus laoreet felis. Integer adipiscing pretium orci. Nulla facilisi. Quisque posuere bibendum purus. Nulla quam mauris, cursus eget, convallis ac, molestie non, enim. Aliquam congue. Quisque sagittis nonummy sapien. Proin molestie sem vitae urna. Maecenas lorem.

As authors may omit details in methods from publication, our AI will look for missing critical information across the 5 most similar protocols.

About PubCompare

Our mission is to provide scientists with the largest repository of trustworthy protocols and intelligent analytical tools, thereby offering them extensive information to design robust protocols aimed at minimizing the risk of failures.

We believe that the most crucial aspect is to grant scientists access to a wide range of reliable sources and new useful tools that surpass human capabilities.

However, we trust in allowing scientists to determine how to construct their own protocols based on this information, as they are the experts in their field.

Ready to get started?

Revolutionizing how scientists
search and build protocols!