Western Blot Analysis of Mitochondrial Dynamics

Western blots were performed as previously described (28 (link)). Blots were incubated with OPA1 antibody (Abcam) diluted 1:1000; DRP1 antibody (Abcam) diluted 1:1000; and GAPDH (Santa Cruz Biotechnology) diluted 1:3000. Signals were detected using horseradish peroxidase-conjugated secondary antibody and Luminata Crescendo Western HRP substrate reagent (Millipore). Blots were imaged using a Chemidoc Imager (Bio-Rad).

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Lima W.F., Murray H.M., Damle S.S., Hart C.E., Hung G., De Hoyos C.L., Liang X.H, & Crooke S.T. (2016). Viable RNaseH1 knockout mice show RNaseH1 is essential for R loop processing, mitochondrial and liver function. Nucleic Acids Research, 44(11), 5299-5312.

Publication 2016

OPA1 antibody DRP1 antibody GAPDH Luminata Crescendo Western HRP substrate reagent Chemidoc Imager

Antibody Gapdh Opa1 Western blots

Corresponding Organization :

Other organizations : Ionis Pharmaceuticals (United States)

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Variable analysis

independent variables

OPA1 antibody dilution (1:1000)
DRP1 antibody dilution (1:1000)
GAPDH antibody dilution (1:3000)

dependent variables

Protein expression levels of OPA1, DRP1, and GAPDH

control variables

Horseradish peroxidase-conjugated secondary antibody
Luminata Crescendo Western HRP substrate reagent (Millipore)

controls

Positive control: Not explicitly mentioned
Negative control: Not explicitly mentioned

Annotations

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