Culturing and Maintaining Prostate Cancer Cell Lines
Commercially available PCa cell lines (RWPE-1, LNCaP, 22Rv1, VCaP, LAPC4, PC3, DU145, NCI-H660, C4-2) were purchased from ATCC and maintained according to ATCC protocols. WCM154 and WCM155 CRPC-NE cell lines have been previously established and were maintained in two-dimensional monolayer culture according to the previously described protocol29 (link). LNCaP-AR cells were a kind gift from Dr. Sawyers and Dr. Mu (Memorial Sloan Kettering Cancer Center) and were cultured as previously described12 (link). MSKCC-PCa3 CRPC-Adeno cells were a kind gift from Dr. Chen (Memorial Sloan Kettering Cancer Center) and were maintained identically to WCM154 and WCM155 cells. All cell lines used and their phenotype are listed in Supplementary Table 4. Cell cultures were regularly tested for Mycoplasma contamination and confirmed to be negative.
Cyrta J., Augspach A., De Filippo M.R., Prandi D., Thienger P., Benelli M., Cooley V., Bareja R., Wilkes D., Chae S.S., Cavaliere P., Dephoure N., Uldry A.C., Lagache S.B., Roma L., Cohen S., Jaquet M., Brandt L.P., Alshalalfa M., Puca L., Sboner A., Feng F., Wang S., Beltran H., Lotan T., Spahn M., Kruithof-de Julio M., Chen Y., Ballman K.V., Demichelis F., Piscuoglio S, & Rubin M.A. (2020). Role of specialized composition of SWI/SNF complexes in prostate cancer lineage plasticity. Nature Communications, 11, 5549.
Other organizations :
Lander Institute, Cornell University, University of Bern, University Hospital of Basel, University of Basel, University of Trento, Hospital of Prato, UCSF Helen Diller Family Comprehensive Cancer Center, Memorial Sloan Kettering Cancer Center, Dana-Farber Cancer Institute, Johns Hopkins Medicine, Johns Hopkins University, Lindenhofspital, University of Duisburg-Essen, Essen University Hospital, University Hospital of Bern
Cell culture protocols maintained according to ATCC and previously described protocols
Cell lines regularly tested for Mycoplasma contamination and confirmed negative
positive controls
Not mentioned
negative controls
Not mentioned
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