Ruthenium Red Staining for TEM

The samples were stained and processed as previously described using ruthenium red [24 (link)]. Briefly, samples were stained with ruthenium red (Sigma-Aldrich, Steinheim, Germany) while in 1% glutaraldehyde, then further processed with the rOTO protocol before embedding in Epon (Serva, Heidelberg, Germany). In addition, 70 nm ultrathin sections of the tissue were analyzed using the JEOL JEM-1400 electron microscope (JEOL, Tokyo, Japan) operating at 80 kV and equipped with a 4K TemCam F416 CMOS camera (Tietz Video and Image Processing Systems GmBH, Gautig).

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Zheng Y., Pierce A.F., Wagner W.L., Khalil H.A., Chen Z., Funaya C., Ackermann M, & Mentzer S.J. (2021). Biomaterial-Assisted Anastomotic Healing: Serosal Adhesion of Pectin Films. Polymers, 13(16), 2811.

Publication 2021

ruthenium red JEOL JEM-1400 electron microscope

Cmos Electron microscope Epon Glutaraldehyde Tissue

Corresponding Organization : Harvard University

Other organizations : University Medical Center of the Johannes Gutenberg University Mainz, Johannes Gutenberg University Mainz

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Variable analysis

independent variables

Ruthenium red staining

dependent variables

Ultrastructural features of the tissue observed using electron microscopy

control variables

1% glutaraldehyde fixation
ROTO protocol
Epon embedding
Tissue sectioning at 70 nm thickness
JEOL JEM-1400 electron microscope operating at 80 kV
4K TemCam F416 CMOS camera

controls

Positive control: Ruthenium red staining

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